GREEN PIGS demonstrate success of UH reproductive science technique

Along with red, green is the color of this holiday season. And bright green is showing up in more than just decorations. In Guangdong province in Southern China, ten transgenic piglets have been born this year–six of them since August–and under a black light, they glow a greenish tint.

A technique developed by reproductive scientists from the University of Hawai`i Mānoa John A. Burns School of Medicine was used to quadruple the success rate at which plasmids from a fluorescent protein in jellyfish DNA were transferred into the embryo of the pig. Drs. Zhenfang Wu and Zicong Li of China’s South Agricultural University have detailed the research that produced the transgenic pigs in an academic manuscript. Dr. Zicong is an alumnus of UH-Mānoa. Also assisting in the manuscript was Dr. Johann Urschitz, an assistant researcher at the UH-Mānoa medical school’s Institute for Biogenesis Research (IBR).

In a video accompanying the research, the pigs–not unlike human children afraid of the dark– begin to squeal when the overhead lights are turned off. A black light then helps illuminate the green tint. The noise is also due in part to the fact that the scientists are opening the pig’s mouth, where the florescent glow is most visible.

The green color simply indicates that the fluorescent genetic material injected into the pig embryos has been incorporated into the animal’s natural make up.

“It’s just a marker to show that we can take a gene that was not originally in the animal and now exists in the animal,” explains Dr. Stefan Moisyadi, veteran bioscientist with the IBR.

Dr. Moisyadi said the animals are not affected by the fluorescent protein and will have the same life span as other pigs. “The green is only a marker to show that’s it’s working easily,” he said.

The ultimate goal is to introduce beneficial genes into larger animals to create less costly and more efficient medicines.

“[For] patients who suffer from hemophilia and they need the blood-clotting enzymes in their blood, we can make those enzymes a lot cheaper in animals rather than a factory that will cost millions of dollars to build,” Dr. Moisyadi said.

The IBR technique involves proprietary pmgenie-3 plasmids conferring active integration during cytoplasmic injection. This technique was also used to produce the world’s first “glowing green rabbits” in Turkey earlier this year. Turkey is expected to announce results of similar research involving sheep in the New Year.

The Institute for Biogenesis Research at the John A. Burns School of Medicine is the home of high-level reproductive research. It is located within the medical school’s department of Anatomy, Biochemistry and Physiology. Its goals include continuing to improve human in vitro fertilization techniques. Its Director is Dr. W. Steven Ward.

The direct link to the Video Clip is at UH MED’s VIMEO CHANNEL.

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